|
| |
Reverse phase purification on the puriFlash 450
using a preparative HPLC column, 30 x 150mm
Part Number: GV3010, Strategy C18-2 10μ, 30 x 150mm
 | | 400mg Intermediate
Gradient
| A: |
Water + 0.1% TFA |
| B: |
Acetonitrile +
0.1% TFA |
0-1 Minute, 10% B
12 Minutes, 70% B
14 Minutes, 70% B
16 Minutes, 100% B |
| Flow Rate: |
40 ml/min |
| UV: |
254 & 280nm |
|
 | | 1.2g Sample Load
2ml Injection, 150mg/ml; Caffeine, Sulfadimethoxine, acetophenone, ketoprofen
Gradient
| A: |
Water +
3% n-PrOH |
| B: |
Acetonitrile +
3% n-PrOH |
0-30 seconds 10% B
10 Minutes, 90% B |
| Flow Rate: |
40 ml/min |
| UV: |
254 & 220nm |
|
 | | 180mg Amide
Gradient
| A: |
2mM NH3 in Water |
| B: |
2mM NH3 in Acetonitrile |
0-3 Minutes, 50% B
20 Minutes, 95% B |
| Flow Rate: |
25 ml/min |
| UV: |
254 & 280nm |
|
|
