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PuriFlash Column General Guidelines
Normal Phase |
| In normal-phase chromatography, the stationary phase is polar and the mobile phase is less polar. The more polar the solvent, the greater the elution strength. To determine optimum conditions, please refer to TLC optimization. Includes: SIHC, SIHP, ALB, ALN, NH2HC, NH2, DEAP, HILIC, DIOL, CN. |
| Use: | It is possible to re-use puriFlash SIHC and SIHP columns by flushing with 100% methanol followed by air. |
| Storage: | Store dry after purging with methanol and air. Do not use hexane as it will soften the polypropylene column hardware. |
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| Reverse Phase | | In reverse phase chromatography, the stationary phase is non-polar or weakly polar and the mobile phase is more polar. A less polar solvent has higher elution strength. Combinations of water with either methanol or acetonitrile are most often used for gradient elution. Compared to normal phase, reverse phase provides higher efficiency and improved selectivity but lower sample loading capacity. Includes: C18HC, C18HP, C18HQ, C18AQ, RPAQ, 302H, X, CN, NH2HC, NH2, MM1, ALB, P6, DEAP. |
| Use: | It is possible to re-use puriFlash reverse phase columns by flushing with 100% organic after each run. |
| Storage: | Remove organic modifiers, buffer salts and flush with 75% Methanol or Acetonitrile in water with end caps in place. |
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| Ion Exchange |
| In ion exchange chromatography, the stationary phase is bonded with ionic functional groups that retain the solute ions of opposite charge. Includes: SCX, SAX, MM1, NH2HC, NH2, DEAP. |
| To Retain: | Anion Exchange - pKa of the target compound < pH < pKa of the sorbent
Cation Exchange - pKa of the sorbent < pH < pKa of the target compound |
| To Elute: | Anion Exchange - pKa of the sorbent < pH < pKa of the target compound
Cation Exchange - pKa of the target compound < pH < pKa of the sorbent |
| Storage: | Remove buffer salts by flushing with water and store in 100% Acetonitrile with end caps in place. |
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