PuriFlash Column Details
Interchim provides a comprehensive range of columns for general purpose and specialty purification.
NOTE: Use PuriFlash Columns menu to the left to jump to column sections.
| Normal Phase, Bare Silica |
| Phase Structure (click to view) |
Type |
Application |
Capacity |
Base Material |
pH Stability |
General Purpose |
UPFP |
Order Info |
 |
SIHC |
Non-ionic polar organic compounds. Methanol stable,
high loading Capacity and high resolution. |
20% |
High Capacity Spherical Silica |
1.5 - 6.5 |
50µ |
15, 25 & 30μ | More... |
|
SIHP |
Non-ionic polar organic compounds. Methanol stable, high separation efficiency at high flow rates. |
10% |
High Performance Spherical Silica |
1.5 - 6.5 |
50μ |
15, 25 & 30μ | More... |
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| Normal Phase, Alumina |
| Phase Structure (click to view) |
Type |
Application |
Capacity |
Base Material |
pH Stability |
General Purpose |
UPFP |
Order Info |
 |
ALB |
Basic compound purification. Eliminate the use of TEA (triethylamine) to avoid streaking or tailing under normal or reverse phase conditions. |
10% |
Granular Basic Alumina |
1 - 12 |
45μ |
|
More... |
 |
ALN |
Acid sensitive compounds that would degrade if exposed to normal phase silica. |
10% |
Granular Neutral Alumina |
1 - 12 |
45μ |
| More.. |
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| Normal Phase, Decolorization |
| Type |
Application |
Capacity |
Base Material |
pH Stability |
Particle Size |
Order Info |
| Carbon |
Decolorization, no exposure to carbon dust |
10% |
Activated Carbon |
1 - 14 |
50μ |
More... |
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| Normal Phase, Dry-Load |
| Type |
Application |
Capacity |
Base Material |
pH Stability |
Particle Size |
Order Info |
| dry-load |
Hand tight, no adapters, stable to 15 bar / 215 PSI. |
NA |
Silica or Celite |
1.5 - 6.5 |
50μ |
More... |
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| Normal Phase, Bonded Silica, NH2 |
| Phase Structure (click to view) |
Type |
Application |
Capacity |
Base Material |
pH Stability |
General Purpose |
UPFP |
Order Info |
 |
NH2HC |
Polar amine compounds with reduced amine-silanol interaction. Suitable for carbohydrates. High Capacity, for higher loading. Eliminate the use of TEA (triethylamine) for basic compounds. |
7% |
High Capacity Spherical Silica |
1.5 - 6.5 |
50μ | |
More... |
|
NH2 |
Polar amine compounds with reduced amine-silanol interaction. Suitable for carbohydrates. Eliminate the use of TEA (triethylamine) for basic compounds. |
5% |
High Performance Spherical Silica |
2 - 6.5 |
50μ | 30μ |
More... |
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| Normal Phase, Bonded Silica, CN |
| Phase Structure (click to view) |
Type |
Application |
Capacity |
Base Material |
pH Stability |
General Purpose |
Order Info |
 |
CN |
Mid range polarity between bare silica and C18. |
6% |
High Performance Spherical Silica |
1.5 - 7 |
50μ |
More... |
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| Normal Phase, Bonded Silica, DIOL |
| Phase Structure (click to view) |
Type |
Application |
Capacity |
Base Material |
pH Stability |
General Purpose |
UPFP |
Order Info |
 |
Diol |
Enhanced selectivity and higher retention for low to medium polarity compounds vs. bare silica. |
6% |
High Performance Spherical Silica |
1.5 - 7 |
50µ | 30µ |
More... |
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| Normal Phase, Bonded Silica, HILIC |
| Phase Structure (click to view) |
Type |
Application |
Capacity |
Base Material |
pH Stability |
UPFP |
Order Info |
 |
HILIC |
Very polar water soluble compounds; dyes, polyphenols, nucleotides and alkaloids using aqueous
solvent systems. Reduce the use of chlorinated solvents using 100% MeOH or H2O. Reduced rotavap time by using less water. |
7% |
High Performance Spherical Silica |
1.5 - 7.5 |
30μ |
More... |
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| Normal Phase, Bonded Silica, DEAP |
| Phase Structure (click to view) |
Type |
Application |
Capacity |
Base Material |
pH Stability |
General Purpose |
Order Info |
 |
DEAP |
DiethylAminoPropyl bonded silica. A less polar alternative to NH2. Compatible with MeOH and water
for use in normal or reverse phase. |
NA |
Granular Silica |
NA |
60μ |
More... |
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| Reverse Phase Bonded Silica |
| Phase Structure (click to view) |
Type |
Application |
Capacity |
Base Material |
pH Stability |
General Purpose |
UPFP |
Order Info |
 |
C18HC |
Non polar and ionic compounds. Fully end-capped. |
3% |
High Capacity
Spherical Silica |
1 - 7.5 |
|
15 & 30μ |
More... |
|
C18HP |
Non polar and ionic compounds. Fully end-capped. |
3% |
High Performance
Spherical Silica |
1 - 7.5 |
50μ |
15 & 30μ |
More... |
|
C18HQ |
Non polar and ionic compounds. Fully end-capped. |
3% |
HPLC Prep Grade, Spherical Silica |
1 - 7.5 |
|
15μ |
More... |
 |
C18AQ |
Mid polar compounds. Start gradient with 100% water. |
3% |
High Performance
Spherical Silica |
1.5 - 7.5 |
|
15 & 30μ |
More... |
| Reverse Phase Bonded Silica |
| Phase Structure (click to view) |
Type |
Application |
Capacity |
Base Material |
pH Stability |
General Purpose |
UPFP |
Order Info |
 |
RPAQ |
Polar compounds. Start gradient with 100% water. |
3% |
High Performance Spherical Silica |
1.5 - 7.5 |
|
15 & 30μ | More.. |
|
DEAP |
DiethylAminoPropyl bonded silica. A less polar alternative to NH2. Compatible with MeOH and water
for use in normal or reverse phase. |
NA |
Granular Silica |
NA |
60μ |
| More.. |
 |
PP-C18T |
Moderately hydrophobic peptides & oligopeptides.
<40 kDa. |
2% |
300Å Spherical Silica |
1.5 - 8 |
|
15 & 30μ | More.. |
|
PT-C18T |
Weak hydrophobic peptides/oligopeptides. <50 kDa. |
2% |
200Å Spherical Silica |
1.5 - 8 |
|
15 & 30μ | More.. |
| Reverse Phase Bonded Silica |
| Phase Structure (click to view) |
Type |
Application |
Capacity |
Base Material |
pH Stability |
General Purpose |
UPFP |
Order Info |
 |
PP-C8 |
Hydrophobic polypeptides & peptides. <80 kDa. |
2% |
300Å Spherical Silica |
2 - 7 |
|
15 & 30μ | More.. |
|
PT-C8 |
Moderately hydrophobic peptides & oligopeptides.
<60 kDa. |
2% |
200Å Spherical Silica |
2 - 7 |
|
15 & 30μ | More.. |
| Reverse Phase Bonded Silica |
| Phase Structure (click to view) |
Type |
Application |
Capacity |
Base Material |
pH Stability |
General Purpose |
UPFP |
Order Info |
 |
PP-C4 |
Hydrophobic proteins & polypeptides 50 - 150 kDa. |
2% |
300Å Spherical Silica |
2 - 7 |
|
15 & 30μ | More.. |
|
PT-C4 |
Hydrophobic peptides & oligopeptides. <60 kDa. |
2% |
200Å Spherical Silica |
2 - 7 |
|
15 & 30μ | More.. |
Back to top | Back to Column Overview | Back to Column Selection Chart | (Silica vs Polymer)
| Reverse Phase, Polymers |
| Phase Structure (click to view) |
Type |
Application |
Capacity |
Base Material |
pH Stability |
General Purpose |
UPFP |
Order Info |
 |
302H |
Hydrophilic/hydrophobic polymer for polar and non polar compounds. MW <3 kDa. High Capacity reverse phase. |
>25% |
SDVB Polymer |
1 - 13 |
70µ | 30µ |
More... |
 |
Atoll X |
Atoll X is a universal polymer for mid & non polar compounds, MW <3 kDa. High Capacity reverse
phase. |
>20% |
SDVB Polymer |
1 - 13 |
40µ | |
More... |
 |
P6 |
Amide functionality for aromatic and natural products; flavonoids, chalkones, anthraquinones, aromatic nitro compounds, DNP amino acids, phenols, carbonic acids, acid amides, sulphonic acids and amides of sulphonic acids and amines. |
5% |
Polyamide |
1 - 8 |
50µ | |
More... |
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| Ion Exchange, Bonded Silica |
| Phase Structure (click to view) |
Type |
Application |
Capacity |
Base Material |
pH Stability |
General Purpose |
UPFP |
Order Info |
 |
SCX |
Sulfonic acid functionality which retains weak basic compounds that have one or more positive charges.
Remove or isolate basic compounds. |
0.3 meq/g |
High Performance Spherical Silica |
1 - 7.5 |
50µ |
|
More... |
 |
SAX |
Quaternary amine functionality which retains strong (pKa <1) or weak acids (pKa >2). |
0.3 meq/g |
Granular Silica |
.51 - 7 |
50µ |
|
More... |
| Ion Exchange, Bonded Silica |
| Phase Structure (click to view) |
Type |
Application |
Capacity |
Base Material |
pH Stability |
General Purpose |
UPFP |
Order Info |
|
DEAP |
DiethylAminoPropyl bonded silica. A less polar alternative to NH2. Compatible with MeOH and water
for use in normal or reverse phase. |
NA |
Granular Silica |
NA |
60μ |
| More.. |
 |
MM1 |
Unique selectivity of basic, charged and non-polar compounds via ion exchange and hydrophobic chains
(SCX/C8). Compounds that possess basic functionality are retained by ion exchange. A washing step with an appropriate pH, elutes ionizable compounds. Retained compounds from hydrophobic bonding are eluted with an organic solvent. |
2% |
High Performance Spherical Silica |
1 - 7.5 |
60μ |
| More.. |
|
NH2HC |
Ethyl group provides separation mechanism. Most often used in ion exchange or normal phase due to its polar nature. |
2% |
High Capacity Spherical Silica |
1.5 - 6.5 |
50μ |
| More.. |
|
NH2 |
Ethyl group provides separation mechanism. Most often used in ion exchange or normal phase due to its polar nature. |
1% |
High Performance Spherical Silica |
2 - 6.5 |
50μ |
30μ | More.. |
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| BioPurification, Reverse Phase |
| Phase Structure (click to view) |
Type |
Application |
Capacity |
Base Material |
pH Stability |
General Purpose |
UPFP |
Order Info |
|
PP-C18T |
Moderately hydrophobic peptides & oligopeptides.
<40 kDa. |
2% |
300Å Spherical Silica |
1.5 - 8 |
|
15 & 30μ | More.. |
|
PT-C18T |
Weak hydrophobic peptides/oligopeptides.
<50 kDa. |
2% |
200Å Spherical Silica |
1.5 - 8 |
|
15 & 30μ | More.. |
|
PP-C8 |
Hydrophobic polypeptides
& peptides. <80 kDa. |
2% |
300Å Spherical Silica |
2 - 7 |
|
15 & 30μ | More.. |
|
PT-C8 |
Moderately hydrophobic peptides & oligopeptides.
<60 kDa. |
2% |
200Å Spherical Silica |
2 - 7 |
|
15 & 30μ | More.. |
|
PP-C4 |
Hydrophobic proteins & polypeptides 50 - 150 kDa. |
2% |
300Å Spherical Silica |
2 - 7 |
|
15 & 30μ | More.. |
|
PT-C4 |
Hydrophobic peptides & oligopeptides. <60 kDa. |
2% |
200Å Spherical Silica |
2 - 7 |
|
15 & 30μ | More.. |
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| BioPurification, Cellulose Media |
| Phase Structure (click to view) |
Application |
Capacity |
Base Material |
pH Stability |
Particle Sizes |
Order Info |
| 90A500 (DEAE) |
Weak anion exchange, intermediate purification of proteins, peptides and enzymes up to 500 kDa. |
>60 mg/ml BSA |
Cellulose Beads |
1 - 13 |
90μ |
More.. |
| 90Q500 (QA) |
Strong anion exchange, intermediate purification of proteins, peptides and enzymes up to 500 kDa. |
>10 mg/ml BSA |
Cellulose Beads |
2 - 12 |
90μ |
More.. |
| 90C500 (CM) |
Weak anion exchange, intermediate purification of proteins, peptides and enzymes up to 500 kDa. |
>70 mg/ml
Lysozyme |
Cellulose Beads |
1 - 13 |
90μ |
More.. |
| 75SO4 (Sulfate) |
Affinity purification of Virus and heparin binding protein up to 3 kDa. |
>3 mg/ml
Lysozyme |
Cellulose Beads |
3 - 12 |
75μ |
More.. |
| 90EL |
Affinity, endotoxin removal up to 2000 kDa. |
480 μg/ml |
Cellulose Beads |
2 - 13 |
90μ |
More.. |
| 75ES |
Affinity, endotoxin removal up to 2 kDa. |
185 μg/ml |
Cellulose Beads |
2 - 13 |
75μ |
More.. |
| 90C4 (butyl) |
Hydrophobic interaction for hydrophobic proteins up to 4000 kDa. |
25 mg/ml
BSA |
Cellulose Beads |
1 - 13 |
90μ |
More.. |
| 90PH (phenyl) |
Hydrophobic interaction for hydrophobic proteins and
enzymes up to 4000 kDa. |
30 mg/ml
BSA |
Cellulose Beads |
1 - 13 |
90μ |
More.. |
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| Natural Products |
| Phase Structure (click to view) |
Type |
Application |
Capacity |
Base Material |
pH Stability |
General Purpose |
UPFP |
Order Info |
 |
P6 |
Amide functionality for aromatic and natural products; flavonoids, chalkones, anthraquinones, aromatic nitro compounds, DNP amino acids, phenols, carbonic acids, acid amides, sulphonic acids and amides of sulphonic acids and amines. |
5% |
Polyamide |
1 - 8 |
50µ | |
More... |
|
CN |
Mid range polarity between bare silica and C18. |
6% |
High Performance Spherical Silica |
1.5 - 7 |
50μ | |
More... |
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|
