UPFP reduces the time and cost per sample from the purification process with increased throughput and confidence. What differentiates UPFP from Flash chromatography is the small particle spherical silica which offers significant benefits over the traditional flash grade silica. UPFP columns greatly improve separation efficiency which allows for higher loading and faster throughput.

This seven minute run uses a 15µ, 25g column at 40 ml/min. The flow is double the rate which is typically considered for a 25g column. Despite the high flow rate, resolution is superior to a standard 40-60µ column and completed in a fraction of the time. See Test conditions here.

Increased pressure is a factor in UPFP, but it is easily managed with columns that are stable to 320 PSI / 22 bar. The puriFlash 430 is rated to 425 PSI / 30 bar. Click here to learn more about UPFP Instruments.

The puriFlash HP 30µ columns operate at the same pressure when compared to conventional 40-60µ flash columns. This is a result of the highly efficient spherical silica improving flow mechanics and permeability.

In the example below, conventional flash grade silica is compared to UPFP silica with the following results: 500% higher loading, 70% less solvent and 300% less collection volume.