Applications | Prep HPLC, Reverse Phase

Reverse phase purification on the puriFlash 450 using a preparative HPLC column.

PuriFlash C18-HQ 10μ, 30 x 150 mm. (Part number PF10C18HQ-150/300)

	400mg Intermediate | In DMSO Gradient: A:  Water + 0.1% TFA B:  Acetonitrile + 0.1% TFA 0-1 Minute, 10% B 12 Minutes, 70% B 14 Minutes, 70% B 16 Minutes, 100% B Flow Rate:  40 ml/min UV:  254 & 280nm
	1.2g Sample Load 2 ml Injection, 150mg/ml, Caffeine, Sulfadimethoxine, acetophenome, ketoprofen in DMSO Gradient: A:  Water + 3% n-PrOH B:  Acetonitrile + 3% n-PrOH 0-30 seconds 10% B 10 Minutes, 90% B Flow Rate:  40 ml/min UV:  254 & 280nm
	180mg Amide Gradient: A:  2mM NH3 in Water B:  2mM NH3 in Acetonitrile 0-3 Minutes, 60% B 20 Minutes, 85% B Flow Rate:  25 ml/min UV:  254 & 280nm