Flash Instruments—Modular Specifications and Options

Purification of synthesis reaction mixtures, natural product extracts, proteins and peptides from mg to gram quantities are easily accomplished with puriFlash instruments. The multi–wavelength UV-DAD (diode array detection) includes real-time full spectra scanning to confirm purity. Detector options include MS, ELSD, RI and Conductivity.

The heart of the instrument is the iQuat gradient HPLC pump which provides a quaternary gradient plus a dedicated purge line (A+B+C+D, i). A ten-second isopropanol purge prepares the flow path for either normal or reverse phase.

Normal Phase

The iQuat gradient pump has 1% gradient accuracy. This allows for shallow gradients without having to premix the mobile phase. 0 – 5% MeOH in DCM over 20 column volumes is accurate and reproducible.

Reverse Phase

Pressure in reverse phase is much higher than normal phase and sample precipitation is also quite common. These pressure spikes present a major compromise with low pressure flash instruments. PuriFlash instruments are stable to 435 PSI/30 bar which support the use of columns packed with 15μ spherical particles. UPFP (Ultra-Performance Flash Purification) columns packed with 15μ particles provide resolution approaching Prep HPLC.

UV-DAD

The UV-Diode Array Detector is suitable for small and large scale purification. A minimum threshold of 1 mAU ensures low sample concentration will be detected and collected. With a range to 50,000 mAU, detector saturation is minimized and ensures no plateau shaped peaks. Collect and/or monitor four wavelengths or scan a range to analyze purity.

Save Hood Space, Time and the Environment

The fume enclosure option permits bench-top installation freeing up hood space for increased productivity. Reduce solvent consumption and run time up to 70% with UPFP (Ultra-Performance Flash Purification).

18.9 x 17.5 x 26.5" (WxDxH)

Standard configurations include an HD touch screen controller, pump, UV-DAD and collector. (Click here for options and specifications)

PuriFlash Standard Configuration

15" HD Touch Screen Controller
iQuat HPLC Pump
UV-DAD 200-400nm
Fraction Collector
4 Racks, 176 x 18mm Tubes