Preparative HPLC Columns
An Interchim prep column will operate at lower pressure which translates into longer column life. The lower pressure is attributed to the removal of fines (<1µ diameter silica particles). The presence of fines in the column will gradually migrate to the outlet frit where they are trapped and cause pressure build up. Fines are removed from the bonded silica prior to packing the column. Compared to other manufacturers’ prep columns an Interchim column will typically exhibit 30 – 40% lower pressure. Examples of lower pressure are found here. In each of the applications pressure never exceeded 30 bar/435 PSI.
|Inner Diameter||Sample Load||Flow Rate|
|21.2mm||20X||20 – 25ml/min|
|30.0mm||30X||30 – 40ml/min|
|50.0mm||100X||100 – 120ml/min|
|75.0mm||250X||200 – 250ml/min|
Linear scale-up is achieved by developing an analytical method which uses the same bonded phase and particle size as the prep column. Alternatively, the analytical method can use the same bonded phase with a smaller particle on a shorter column.
After optimizing the separation on the analytical column, the appropriate prep column is selected based upon the desired sample load.
Small Molecule Purification
Uptisphere Strategy provides linear scale up from UHPLC to Prep. The high surface area silica (425 m2/g, 100Å) is available in a range of particle sizes to suit any budget: 2.21, 31, 51, 101 and 151
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|C18-3||22%||Multi-Step||1.0 – 12.0||Strong retention of non-polar compounds and basic drugs at high pH.|
|C18-HQ||19%||Multi-Step||1.0 – 10.0||High capacity loading for mid and non-polar compounds (formerly C18-2).|
|C18-RP||16%||Multi-Step||1.5 – 8.0||Mid- and non-polar compounds under acidic or basic conditions.|
|C18-AQ||14%||One-Step||2.0 – 7.5||Stable in 100% aqueous mobile phase for mid- and non-polar compounds.|
|Phenyl-C4||12%||One-Step||2.0 – 7.0||Very selective for cyclic aromatic and mid-polar compounds.|
|Hilic HIT||N/A||One-Step||1.5 – 7.0||Aqueous normal phase (ANP) for highly polar compounds. Typical mobile phase: water/ACN (>70%).|
|Hilic HIA||N/A||One-Step||2.0 – 7.0||Aqueous normal phase (ANP) for highly polar compounds. Typical mobile phase: water/ACN (>70%).|
|Si||N/A||N/A||1.5 – 7.0||Normal phase separation of non-ionic, polar compounds.|
Uptisphere X-Series is available in several pore sizes and bonded phases to address the wide range of selectivity required for biopurification.
|Phase||% C||Bonding||Pore Size||pH Range||
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|C18-OD2||20%||Polyfunctional||130Å||1.0 – 13.0||Biopolymers <10 kDa, 130Å silica.|
|C18||14%||Polyfunctional||220Å||1.0 – 13.0||Biopolymers <30 kDa, 220Å silica.|
|C18-AQ||14%||Polyfunctional||220Å||1.0 – 10.0||Biopolymers <30 kDa, 220Å silica, stable in 100% water.|
|C8||8%||Polyfunctional||220Å||1.0 – 13.0||Biopolymers <50 kDa, 220Å silica.|
|C4||6%||Polyfunctional||220Å||1.0 – 13.0||Biopolymers <60 kDa, 220Å silica.|
|C18-WOD||10%||Monofunctional||300Å||1.5 – 7.0||Weakly hydrophobic biopolymers <50 kDa, 300Å silica.|
|C4-WC4||4%||Monofunctional||300Å||2.0 – 7.0||Hydrophobic biopolymers 50 - 150 kDa, 300Å silica.|
|C4-WD4||4%||Polyfunctional||300Å||1.5 – 8.0||Strongly hydrophobic biopolymers 50 - 150 kDa, 300Å silica.|
|C4-WT4||3%||Trifunctional||300Å||1.5 – 8.0||Strongly hydrophobic biopolymers 50 - 150 kDa, 300Å silica.|