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Preparative HPLC

The puriFlash 450 and 4100 can be used as a traditional flash instrument or as a preparative HPLC. Similar to a preparative HPLC, the sample is loaded into the loop of the injection valve. A 2ml loop is standard with options up to 10ml.

Reverse phase purification on the puriFlash 450 using a preparative HPLC column,
Strategy C18-2 10µ , 30 x 150mm. (Part Number GV3010)
Reverse phase purification, 400mg Intermediate

400mg Intermediate Gradient
A: Water + 0.1% TFA
B: Acetonitrile + 0.1% TFA
0-1 Minute, 10% B
12 Minutes, 70% B
14 Minutes, 70% B
16 Minutes, 100% B
40 ml/min.
UV @ 254 & 280nm
Reverse phase purification, 1.2g Sample Load

1.2g Sample Load
2ml Injection, 150mg/ml; Caffeine,
Sulfadimethoxine, acetophenone,
ketoprofen
Gradient
A: Water + 3% n-PrOH
B: Acetonitrile + 3% n-PrOH
0 -:30 seconds 10% B
10 Minutes, 90% B
40 ml/min.
UV @ 254 & 220nm

Reverse phase purification, 180mg Amide

180mg Amide
Gradient
A: 2mM NH3 in Water
B: 2mM NH3 in Acetonitrile
0-3 Minutes, 50% B
20 Minutes, 95% B
25 ml/min.
UV @ 254 & 280nm

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