Test Conditions
| Column | Flow | Pressure | Gradient profile (Column Volume) | Sample Load |
| 15µ puriFlash Si HP, 25g | 40 ml/min | 30 psi | 0 - 4.8: 0-20% B, 4.8 - 5.6: 20% B | 2ml |
| 40-60µ puriFlash Si IR, 25g | 20 ml/min | 15 psi | 0 - 9.6: 0 - 20% B* | 2ml |
Solvents A: Hexane B: Ethyl acetate
Sample: 500mg/ml Dimethyl phthalate, Diethyl phthalate, Dibutyl phthalate
Wavelength: 254nm
*Gradient extended to provide comparable resolution